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KMID : 0379119980260010091
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Korean Journal of Mycology
1998 Volume.26 No. 1 p.91 ~ p.96
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Preservation of Mushroom Cultures in Sterile Distilled Water
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김창진/Kim, Chang Jin
이동훈/신광수/Lee, Dong Hoon/Shin, Kwang Soo
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Abstract
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Optimal conditions for the preservation of mushroom cultures in sterile distilled water were investigated. Mycelial disks from 12 mushroom cultures were preserved at room temperature, 5℃, - 20℃, and - 75℃ using sterile water and 6 cryoprotectants for 12 months. The maximal viability of mycelial disks was observed at room temperature and 5℃ with the value of 91.7%. At - 75℃, the viability was highest in polyethylene glycol (PEG) and lowest in 10% dimethyl sulfoxide (DMSO). The average mycelial growth was highest for disks preserved at 75℃ in PEG. PEG was the most suitable for the preservation at - 75℃, although this chemical has rarely been used as a cryoprotectant in fungal culture preservation. The laccase isozyme patterns of the preserved isolate of H. ferrugineum were not changed during preservation at 5℃ in sterile distilled water as compared to that of slant culture.
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